Sunday, June 30, 2013

Triche looking on bright side heading into NBA Summer League

This section displays all of the articles related to sports published in the past 10 days.

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Android 4.3 leaks for 'Google Play edition' Galaxy S4

 

4.3 leak

The next version of Jelly Bean has leaked for the 'Google Play edition' GS4 — and there's a port for the European GS4 model already

Android 4.3 Jelly Bean isn't even official yet, but already a leaked build has appeared for the Samsung Galaxy S4 "Google Play edition." The pre-release build was uploaded by Samsung fansite SamMobile in its original form, and in the form of a custom ROM for the European Galaxy S4 — GT-i9505G.

The initial batch of screnshots from the ROM (build number JWR66N) doesn't show any major differences from version 4.2.2, however we'll have to reserve judgment until we've played with the software for ourselves. For the moment, however, 4.3 looks to be a relatiely minor bump up from 4.2.

We'll bring you more coverage of this story as it unfolds.

Source: SamMobile (1, 2)

 

    


Source: http://feedproxy.google.com/~r/androidcentral/~3/bRY-XpsFFHM/story01.htm

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Saturday, June 29, 2013

How to Meet an Online Friend in Real Life Without It Being Awkward

How to Meet an Online Friend in Real Life Without It Being Awkward

You're not looking to bang every person you meet online. Sometimes, you're just looking for friends. But somehow, meeting someone you know online platonically has become a far more awkward endeavor than a random OKCupid date. You know her but you dont know her. Do you shake hands? Do you hug? Do you do that open-palmed half-wave? God forbid she goes for the hug and you go for the handshake like you're in some jerking, uncoordinated, chest-poking dance.

We know how to navigate these waters with dates we picked up online, but digital friendships don't always translate seamlessly to the real world. And yet increasingly, internet is how we meet people now. So you better be able to ride out that unavoidable initial awkwardness.

I've made some of my closest friends on the internet. In fact, next month I'll be moving in with someone I met on Twitter. But before we consummated our friendship in real life (minds out of the incognito browsers, children), we had fairly skewed ideas of what to expect. "I was nervous to meet you because we had so much in common," she told me, reasonably. "I was suspicious. Like, what if you were a bot?" I was just afraid her jokes would be corny.

That looming uncertainty of what someone you already know is really like is where a lot of the nerves come from. Plus, there's the very normal desire to make a good first impression. But there are a few easy ways to make the butterflies in your stomach chill out a little bit.

Meet in a group setting. Do you have a few people you know from the internet you'd like to meet? Why not all meet at once? If you're going to be awkward, at least be awkward together. You'd all have to be trying very deliberately to achieve much awkward silence between the five of you. (Though god help you if you do.)

Meet somewhere public. As with Craigslist transactions and drug deals, you'll want to meet somewhere with other people around. A bar is the most obvious destination, because there will at least be a bartender there and there's a clearcut escape route. Plus, booze!

Bring a friend for support. The buddy system isn't a bad idea, either. Sometimes it helps to have another mouth around to keep the conversation going. And again, it provides you with an easy out.

I've actually done real life meetups quite a bit. One that stands out happened in February with a bunch of media types at a dark bar in Brooklyn that serves kitschy beach drinks. We decided, Hey we jabber at each other all day through our keyboards, why not make it honest and actually, you know hang out? This is sort of an extraordinary circumstance, but everything came together perfectly. It started with a few people who knew each other from Twitter and whatnot and wanted to have a drink together, and the word sort of spread to others who wanted to join in on the fun IRL. It was great! And all the awkwardness was avoided thanks to the group setting (and the drinks).

The beauty of the whole thing is that we never would have met were it not for the internet. Not every situation is so ideal, but there's no reason your internet friends can't be real world friends too.

Image credit: Shutterstock/Everett Collection

Source: http://gizmodo.com/how-to-meet-an-online-friend-in-real-life-without-it-be-610568297

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Kerry meets with Palestinian leader

AMMAN, Jordan (AP) ? U.S. Secretary of State John Kerry was meeting Friday with Palestinian President Mahmoud Abbas and planned to return to Jerusalem for more talks with the Israelis as he continues his latest round of Mideast diplomacy in an effort to get both sides back to the negotiating table.

Kerry was using Amman as a base for the talks. He drove to Jerusalem Thursday night for a dinner meeting with Israeli Prime Minister Benjamin Netanyahu that lasted about four hours.

He and his delegation returned to Amman early Friday. Around noon, he drove to a diplomatic area of Amman for a luncheon with Abbas.

"It's good to be back and I look forward to our conversation. We have a lot to talk about, obviously, as you know," Kerry told Abbas.

As reporters and photographers were ushered out of the meeting, Kerry turned to Abbas and said: "We had a good long meeting." It was an apparent reference to his meeting with Netanyahu the night before. "We're going back."

State Department officials have said little about the details of the talks. It was clear from the travel schedule given to the delegation that Kerry was going to go back to Jerusalem, but State Department officials wouldn't confirm the return trip.

It, however, was one of the first things Kerry told Abbas as the two men sat down to talk.

Kerry, who is on a two-week swing through the Mideast and Asia, left Amman on Thursday evening in a convoy of nearly a dozen vehicles for the roughly 90-minute drive to Jerusalem. A Jordanian military helicopter flew over his convoy during the trip, according to a reporter who was allowed to make the trip with Kerry and his delegation.

Netanyahu was about an hour late, apparently telling Kerry that he was delayed because he had been attending a graduation ceremony for Israeli military pilots. They talked mostly one-on-one, but advisers also were present for some of the discussion, which began around 9:30 p.m. local time in a suite at a hotel in Jerusalem and ended around 1:30 a.m. Friday.

The State Department released the dinner menu ? fish ceviche and a main course of red tuna and sea bream over lentils and mushrooms ? but offered no detailed information about their talks. The State Department said only that the two had a "productive, in-depth and wide-ranging conversation" and that Kerry reiterated his commitment to working with all parties to achieve a two-state solution.

There was no readout from the Israelis.

State Department officials say Kerry will continue to try to find common ground between the two sides that would lead to a re-launching of negotiations. On this trip, Kerry is trying to pin down precisely what conditions Abbas and Netanyahu have for resuming talks and perhaps discuss confidence-building measures.

Source: http://news.yahoo.com/kerry-meets-palestinian-leader-102416211.html

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Friday, June 28, 2013

Google developing Android videogame console: report

SAN FRANCISCO (Reuters) - Google Inc is developing a videogame console and a wristwatch based on its Android operating system, according to a report in the Wall Street Journal.

The devices could be available as soon as this Fall, the report, which cited anonymous sources, said.

Google, the world's No.1 Web search engine, is also working on a revamped version of the Nexus Q music-streaming device, the report said. Google unveiled the Nexus Q in June 2012, but never released the product, which received critical reviews.

Google is increasingly involved in the hardware business as it seeks to better compete against iPhone-maker Apple Inc. It acquired mobile phone company Motorola Mobility last year and Google is currently testing a wearable computing device known as Google Glass.

Google's Android operating system is the world's most popular mobile software, featured on three out of every four smartphones sold. A video game console could provide a significant opportunity for Google to expand Android's reach beyond its stronghold in smartphones and tablets.

Google was not immediately available for comment.

(Reporting By Alexei Oreskovic. Editing by Andre Grenon)

Source: http://news.yahoo.com/google-developing-android-videogame-console-report-230612684.html

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Video: Prep Your Retirement Portfolio

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Source: http://www.nbcnews.com/video/cnbc/52344902/

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Thursday, June 27, 2013

Nanny Cam Video Captures Brutal Home Invasion in New Jersey

Source: http://www.thehollywoodgossip.com/2013/06/nanny-cam-video-captures-brutal-home-invasion-in-new-jersey/

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Patriots Tight End Aaron Hernandez Arrested & Dropped From Team (VIDEO)

Patriots Tight End Aaron Hernandez Arrested & Dropped From Team (VIDEO)

Aaron Hernandez cut from PatriotsNew England Patriots football player Aaron Hernandez was dropped from the NFL team just after he was arrested by police at his home and taken away in handcuffs. Hernandez had been questioned and his home was searched in connection with an investigation into the shooting death of a young man, Odin Lloyd, who was found ...

Patriots Tight End Aaron Hernandez Arrested & Dropped From Team (VIDEO) Stupid Celebrities Gossip Stupid Celebrities Gossip News

Source: http://stupidcelebrities.net/2013/06/patriots-tight-end-aaron-hernandez-arrested-dropped-from-team-video/

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Mumford & Sons Set New Tour Dates As Bassist Maintains 'Steady Recovery'

Keyboardist Ben Lovett confirms that Ted Dwane will take the stage on Sunday in the U.K. after undergoing brain surgery.
By Jocelyn Vena

Source: http://www.mtv.com/news/articles/1709527/mumford-sons-tour-dates.jhtml

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Saturday, June 22, 2013

How did a third radiation belt appear in the Earth's upper atmosphere?

June 20, 2013 ? Since the discovery of the Van Allen radiation belts in in Earth's upper atmosphere in 1958, space scientists have believed that these belts consisted of two doughnut-shaped rings of highly charged particles -- an inner ring of high-energy electrons and energetic positive ions, and an outer ring of high-energy electrons.

However, in February of this year, a team of scientists reported in the journal Science the surprising discovery of a previously unknown third radiation ring. This narrow ring had briefly circled Earth between the inner and outer rings in September 2012 and then almost completely disappeared.

How did this temporary radiation belt appear and dissipate?

In new research, the radiation belt group in the UCLA Department of Atmospheric and Oceanic Sciences explains the development of this third belt and its decay over a period of slightly more than four weeks. The research is available in the online edition of the journal Geophysical Research Letters and will be published in an upcoming print edition.

By performing a "quantitative treatment of the scattering of relativistic electrons by electromagnetic whistler-mode waves inside the dense plasmasphere," the investigators were able to account for the "distinctively slow decay of the injected relativistic electron flux" and demonstrate why this unusual third radiation belt is observed only at energies above 2 mega-electron-volts.

Understanding the processes that control the formation and ultimate loss of such relativistic electrons is a primary science objective of the NASA Van Allen Probe Mission and has important practical applications, because the enormous amounts of radiation the Van Allen belts generate can pose a significant hazard to satellites and spacecraft, as well to astronauts performing activities outside a spacecraft.

The current research was funded by the NASA, which launched the twin Van Allen probes in the summer of 2012.

The lead author of the research is Richard Thorne, a UCLA professor of atmospheric and oceanic sciences, who was a co-author of the Feb. 28 research paper in Science. Co-authors of the new research include Wen Li, a graduate student who works in Thorne's laboratory; Binbin Ni, a postdoctoral scholar who works in Thorne's laboratory; Jacob Bortnik, a researcher with the UCLA Department of Atmospheric and Oceanic Sciences; Daniel Baker, a professor at the University of Colorado's Laboratory for Atmospheric and Space Physics and lead author of the February Science paper; and Vassilis Angelopoulos, a UCLA professor of Earth and space sciences.

Source: http://feeds.sciencedaily.com/~r/sciencedaily/top_news/~3/rDLN-kvGG3Y/130620162840.htm

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Friday, June 21, 2013

Transistor made from just one molecular monolayer made to work on computer chip

June 20, 2013 ? Electronic components built from single molecules using chemical synthesis could pave the way for smaller, faster and more green and sustainable electronic devices. Now for the first time, a transistor made from just one molecular monolayer has been made to work where it really counts. On a computer chip.

The molecular integrated circuit was created by a group of chemists and physicists from the Department of Chemistry Nano-Science Center at the University of Copenhagen and Chinese Academy of Sciences, Beijing. Their discovery has just been published online in the periodical Advanced Materials. The breakthrough was made possible through an innovative use of the two dimensional carbon material graphene.

First step towards integrated molecular circuit

Kasper N?rgaard is an associate professor in chemistry at the University of Copenhagen. He believes that the first advantage of the newly developed graphene chip will be to ease the testing of coming molecular electronic components. But he is also confident, that it represents a first step towards proper integrated molecular circuits.

"Graphene has some very interesting properties, which cannot be matched by any other material.

What we have shown for the first time is that it's possible to integrate a functional component on a graphene chip. I honestly feel this is front page news," says N?rgaard.

See through sandwich central to function

The molecular computer chip is a sandwich built with one layer of gold, one of molecular components and one of the extremely thin carbon material graphene. The molecular transistor in the sandwich is switched on and of using a light impulse so one of the peculiar properties of graphene is highly useful. Even though graphene is made of carbon, it's almost completely translucent.

Environmentally important. Strategically vital

The hunt for transistors, wires, contacts and other electronic components made from single molecules has had researchers working night and day. Unlike traditional components they are expected to require no heavy metals and rare earth elements. So they should be cheaper as well as less harmful to earth, water and animals. Unfortunately it has been fiendishly difficult to test how well these functional molecules work. Until now.

The luck of the draw

Previously the testing of the microscopic components had researchers resort to a method best compared to a lottery. In order to check whether or not a newly minted molecule would conduct or break a current, they had to practically dump a beakerfull of molecules between two live wires, hoping that at least one molecule had landed so that it closed the circuit.

Lottery method supplanted by precision placement

Using the new graphene chip researchers can now place their molecules with great precision. This makes it faster and easier to test the functionality of molecular wires, contacts and diodes so that chemists will know in no time whether they need to get back to their beakers to develop new functional molecules, explains N?rgaard.

"We've made a design, that'll hold many different types of molecule" he says and goes on: "Because the graphene scaffold is closer to real chipdesign it does make it easier to test components, but of course it's also a step on the road to making a real integrated circuit using molecular components. And we must not lose sight of the fact that molecular components do have to end up in an integrated circuit, if they are going to be any use at all in real life."

The work has been supported by Danish Chinese Center for Molecular Nano-Electronics and financed by the Danish National Research Foundation, the European Union 7th framework for research (FP7) and by The Lundbeck Foundation.

Source: http://feeds.sciencedaily.com/~r/sciencedaily/top_news/top_science/~3/vz0WSRRz7WE/130620071525.htm

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Senators closing in on border security compromise

WASHINGTON (AP) ? White House-backed immigration legislation is gaining momentum in the Senate, where key lawmakers say they are closing in on a bipartisan compromise to spend tens of billions of dollars stiffening the bill's border security requirements without delaying legalization for millions living in the country unlawfully.

"This is a key moment in the effort to pass this bill. This is sort of the defining 24 to 36 hours," Sen. Lindsey Graham, R-S.C., said Wednesday night after a day of private talks.

Under the emerging compromise, the government would grant legal status to immigrants living in the United States unlawfully at the same time the additional security was being put into place. Green cards, which signify permanent residency status, would be withheld until the security steps were complete.

Officials described a so-called border surge that envisions doubling the size of the Border Patrol with 20,000 new agents, constructing hundreds of miles of additional fencing along the border with Mexico and purchasing new surveillance drones to track would-be illegal border crossers. The cost of the additional agents alone was put at $30 billion over a decade.

Other details were not immediately available, although it was expected that modifications to the bill would range far beyond border security provisions. The changes under consideration were the result of negotiations involving the bipartisan Gang of Eight who drafted the bill and Republicans seeking alterations before they would commit to voting for it.

The officials spoke on the condition of anonymity because the talks were private.

If agreed to, the changes could clear the way for a strong bipartisan vote within a few days to pass the measure that sits atop President Barack Obama's second-term domestic agenda.

The developments came as Democrats who met with House Speaker John Boehner on Wednesday quoted him as saying he expected the House to pass its own version of an immigration bill this summer and Congress to have a final compromise by year's end.

Boehner, R-Ohio, already has said the legislation that goes to the House in the next month or two will not include a pathway to citizenship for immigrants in the United States illegally.

Precise details of the pending agreement in the Senate were unavailable, but Sen. John Hoeven, R-N.D., said it involved a major increase of resources to the border, including more manpower, fencing and technology. The underlying legislation already envisions more border agents; additional fencing along the U.S-Mexico border; surveillance drones; a requirement for employers to verify the legal status of potential workers; and a biometric system to track foreigners who enter and leave the United States at air and seaports and by land.

It was unclear what other portions of the legislation might be changed. There is pressure from some Republicans to make sure no federal benefits go to immigrants who are in the country illegally, at least until they become citizens.

"Our whole effort has been to build a bipartisan group that will support the bill," said Hoeven, who has not yet stated a position on the legislation. "That's what this is all about, and it's focused on border security."

Sen. Chuck Schumer, D-N.Y., one of the bill's most prominent supporters, said discussions with Republicans "have been really productive. We've made a lot of progress in the last 24 hours. Now we have some vetting to do with our respective allies."

The potential compromise came into focus one day after the Congressional Budget Office jolted lawmakers with an estimate saying that as drafted, the legislation would fail to prevent a steady increase in the future in the number of residents living in the United States illegally.

The estimate appeared to give added credibility to Republicans who have been pressing Democrats to toughen the border security provisions already written into the bill. Schumer and Sen. Bob Menendez, D-N.J., met at midday with Graham, Hoeven and Sen. Bob Corker, R-Tenn. The Democrats and Graham are part of Gang of Eight.

If ratified, the compromise would mark concessions on both sides.

Some Republicans have been unwilling to support a bill that grants legal status to immigrants in the country illegally until the government certifies that the border security steps have achieved 90 percent effectiveness in stopping would-be border crossers.

On the other hand, Democrats have opposed Republican proposals to make legalization contingent on success in closing the border to illegal crossings. Under the legislation as drafted, legalization could begin as soon as a security plan was drafted, but a 10-year wait is required for a green card.

One plan to change that was sidetracked on a vote of 61-37.

Sen. Rand Paul, R-Ky., said his proposal would require Congress to vote annually for five years on whether the border is secure. If lawmakers decide it is not, "then the processing of undocumented workers stops until" it is, he said. The decision would be made based on numerous factors, including progress toward completion of a double-layered fence along the U.S.-Mexico border and toward a goal of 95 percent capture of illegal entrants. A system to track the border comings and goings of foreigners is also required.

While the public debate was taking place, lawmakers involved in the private talks expressed optimism.

Across the Capitol, House Republican leaders sought to present a friendlier face to Hispanics ? a group that gave Obama more than 70 percent support in last year's presidential election.

Boehner met with the Democratic-dominated Congressional Hispanic Caucus, while rank-and-file members of his party reviewed areas of agreement with Latino religious leaders.

"It's a conversation Republicans want to have," Rep. Cathy McMorris Rodgers, R-Wash., said later at a news conference outside the Capitol.

Separately, the House Judiciary Committee approved legislation creating a program allowing farm workers to come to the United States to take temporary jobs in the United States.

The measure is one of several that the panel is considering in the final weeks of June as part of a piece-by-piece approach to immigration rather than the all-in-one bill that Senate is considering.

___

Associated Press writer Laurie Kellman contributed to this report.

Source: http://news.yahoo.com/senators-closing-border-security-compromise-071234419.html

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Thursday, June 20, 2013

Superman Needs More Time

Denver Nuggets head coach George Karl reacts against the Golden State Warriors during Game 4 of their NBA Western Division quarter-final basketball playoff game in Oakland, California April 28, 2013.

George Karl was fired this year as coach of the Denver Nuggets after leading the team to the playoffs.

Photo by Robert Galbraith/Reuters

What does the recent mass firing of National Basketball Association coaches have to do with the rapid turnover of school chiefs? Each illustrates our craving for instant gratification?and each comes at a high cost.

A dozen NBA coaches (out of 30) have gotten the boot in recent weeks?and not only those whose teams compiled losing records. Five coaches whose teams made the playoffs received pink slips. That?s ?stupid,? says the Denver Nuggets? George Karl, who, despite being named the coach of the year, was also fired. Who can disagree? It makes no sense to fire a coach with a record of solid accomplishment.

The same unhappy pattern holds true for public school systems, where the average tenure of an urban superintendent is less than three years. The storyline is all too familiar. A new school chief is hired amid much fanfare and sky-high expectations. Steady improvement won?t do. Unless there?s a dramatic change in fortunes, the grumbling starts, and soon afterward the superintendent is looking for a new job. That?s a sure prescription for failure, since there?s no way that a school chief, however talented or inspiring, can make a lasting difference in such a short time.

In researching my book Improbable Scholars: The Rebirth of a Great American School System and a Blueprint for America?s Schools, I found that successful school districts were far more willing to give their superintendents a real chance to prove themselves.

In these districts, the political leadership didn?t expect Superman to show up. They didn?t press the panic button when test scores didn?t go through the roof, nor did they make perform-or-else demands. School officials didn?t feel the unceasing pressure that, in many communities, has led to widespread cheating to inflate students? test scores.

The most effective superintendents have remained on the job for a decade or more. The resulting stability has given them the chance to build a school culture that joins high expectations with a sense of trust and shared mission. That?s a foundation on which their successors can build.

Improbable Scholars is replete with success stories?here, in capsule form, are two of them.

The schools in Union City, N.J., a poor Latino immigrant community, used to be the state?s laughing stock. No more. After years of instability and a threatened state takeover, the district adopted a slow-and-steady approach. Union City has had just two superintendents in a quarter century. Now it boasts world-class preschools, a rigorous district-wide curriculum (critical in poor communities, where families frequently move during the school year), assessments that are used to pinpoint help for struggling students, mentoring and coaching for teachers, who work closely with one another, and a nationally recognized bilingual program that enables teen immigrants, who arrive in the U.S. with a bare-bones education, to graduate in four years. Students? scores on the state?s high-stakes test have come to approximate the statewide average, and the graduation rate is 90 percent, which is 15 percent higher than the national average.

Montgomery County, Md., the 17th largest school system in the nation, used to operate what amounted to two districts, separate and unequal. While students from well-to-do families excelled, a few miles away, kids living in mostly poor and minority neighborhoods dropped out in droves. The school board had booted out several superintendents. Then in 1998 the board picked Jerry Weast to lead the district?and gave him a decent chance. During his 12-year tenure, Weast turned the situation around. He poured resources into the less wealthy schools, and students? performance steadily improved. Meanwhile, academic standards rose district-wide, with a new push to expand enrollment in eighth-grade algebra (a marker for college enrollment) and advanced placement classes. Students in the well-off neighborhood schools did better. Meanwhile, new community liaisons reached out to parents. The teachers union, invited to discuss policy and not just salaries, went from adversary to partner. Across the district graduation rates rose, and the achievement gap between the schools with poor and affluent populations keeps shrinking.

Longevity on the job doesn?t guarantee results, of course, and some superintendents deserve to be sacked. But I don?t know of a single instance in which a new school chief has transformed a district in the short grace period they?re typically allotted. Schools are simply too complex, and the task of embedding a we-can-do-it culture too daunting, to be accomplished overnight.

Much the same holds true for coaches in the NBA. The two teams in this year?s finals, the San Antonio Spurs and the Miami Heat, remain avatars of stability. In Miami, Pat Riley has been at the helm, as coach or general manager, for 18 years. San Antonio has had the same coach, Greg Popovich, for 17 years. "The continuity breeds trust, it breeds camaraderie, it breeds a feeling of responsibility that each member holds towards the other,? Popovich told an AP reporter, ?the ability to be excited for each other's success, not to develop territory and walls, but to stay participatory. To be able to discuss, to argue and come out at the end on the same page with the same passion and the same goals.?

"And I think without continuity that's pretty impossible,? the coach added, ?because all the immediate tendencies of instant success starts to take over and that just breeds failure." He could have been talking about America?s schools.

Source: http://www.slate.com/articles/news_and_politics/science/2013/06/firing_nba_coaches_and_school_superintendents_too_quickly_is_a_bad_thing.html

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Wednesday, June 19, 2013

100K protesters flood Brazilian streets

SAO PAULO (AP) -- In some of the biggest protests since the end of Brazil's 1964-85 dictatorship, demonstrations have spread across this continent-sized country and united people from all walks of life behind frustrations over poor transportation, health services, education and security despite a heavy tax burden.

More than 100,000 people were in the streets Monday for largely peaceful protests in at least eight big cities. They were in large part motivated by widespread images of Sao Paulo police last week beating demonstrators and firing rubber bullets into groups during a march that drew 5,000.

There was some violence, with police and protesters clashing in Rio de Janeiro, Porto Alegre and Belo Horizonte. The newspaper O Globo, citing Rio state security officials, said at least 20 officers and 10 protesters were injured there.

Monday's protests come after the opening matches of soccer's Confederations Cup over the weekend, just one month before a papal visit, a year before the World Cup and three years ahead of the 2016 Olympics in Rio de Janeiro. The unrest is raising some security concerns, especially after the earlier protests produced injury-causing clashes with police.

In Sao Paulo, Brazil's economic hub, at least 65,000 protesters gathered Monday at a small, treeless plaza then broke into three directions in a Carnival atmosphere, with drummers beating out samba rhythms as people chanted anti-corruption jingles. They also railed against the matter that sparked the first protests last week ? a 10-cent hike in bus and subway fares.

Thousands of protesters in the capital, Brasilia, peacefully marched on Congress. Dozens scrambled up a ramp to a low-lying roof, clasping hands and raising their arms, the light from below sending their elongated shadows onto the structure's large, hallmark upward-turned bowl designed by famed architect Oscar Niemeyer. Some congressional windows were broken, but police did not use force to contain the damage.

"This is a communal cry saying: 'We're not satisfied,'" Maria Claudia Cardoso said on a Sao Paulo avenue, taking turns waving a sign reading "#revolution" with her 16-year-old son, Fernando, as protesters streamed by.

"We're massacred by the government's taxes ? yet when we leave home in the morning to go to work, we don't know if we'll make it home alive because of the violence," she added. "We don't have good schools for our kids. Our hospitals are in awful shape. Corruption is rife. These protests will make history and wake our politicians up to the fact that we're not taking it anymore!"

Protest leaders went to pains to tell marchers that damaging public or private property would only hurt their cause. In Sao Paulo, sentiments were at first against the protests last week after windows were broken and buildings spray painted during the demonstrations.

Police, too, changed tactics. In Sao Paulo, commanders said publicly before the protest they would try to avoid violence, but warned they could resort to force if protesters destroyed property. During the first hours of the march that continued into the night there was barely any perceptible police presence.

The Sao Paulo march itself was a family affair: A group of mothers received a rousing cheer when they arrived at the plaza where the march began, brandishing signs that read "Mothers Who Care Show Support."

"I'm here to make sure police don't hurt these kids," said Sandra Amalfe, whose 16-year-old daughter chatted with friends nearby. "We need better education, hospitals and security ? not billions spent on the World Cup."

Officers in Rio fired tear gas and rubber bullets when a group of protesters invaded the state legislative assembly and hurled rocks and flares at police. But most of the tens of thousands who protested in Rio did so peacefully, many of them dressed in white and brandishing placards and banners.

In Belo Horizonte, police estimated about 20,000 people took part in a peaceful protest before a Confederations Cup match between Tahiti and Nigeria. Earlier in the day, demonstrators erected several barricades of burning tires on a nearby highway, disrupting traffic.

Protests also were reported in Curitiba, Belem and Salvador.

Marcos Lobo, a 45-year-old music producer who joined the protest in Sao Paulo, said the actions of police during earlier demonstrations persuaded him to come out Monday.

"I thought they (the protests) were infantile at first because of my preconceived notions," Lobo said. "Then I saw the aggression."

Another protester, Manoela Chiabai, said she wanted to express her dissatisfaction with the status quo.

"Everything in Brazil is a mess. There is no education, health care ? no security. The government doesn't care," the 26-year-old photographer said. "We're a rich country with a lot of potential but the money doesn't go to those who need it most."

In a brief statement, President Dilma Rousseff, who faces re-election next year and whose popularity rating recently dipped for the first time in her presidency, acknowledged the protests, saying: "Peaceful demonstrations are legitimate and part of democracy. It is natural for young people to demonstrate."

Brazilians have long accepted malfeasance as a cost of doing business, whether in business or receiving public services. Brazilian government loses more than $47 billion each year to undeclared tax revenue, vanished public money and other widespread corruption, according to the Federation of Industries of Sao Paulo business group.

But in the last decade, about 40 million Brazilians have moved into the middle class and they have begun to demand more from government. Many are angry that billions of dollars in public funds are being spent to host the 2014 World Cup and 2016 Olympics while few improvements are made elsewhere.

___

Associated Press writers Jenny Barchfield in Rio de Janeiro, Marco Sibaja in Brasilia and Jill Langlois in Sao Paulo contributed to this report.

Source: http://news.yahoo.com/100k-protesters-flood-brazilian-streets-120128585.html

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AT&T and Goal Zero deploy solar-powered Street Charge stations in NYC, we go hands-on (video)

AT&T and Goal Zero deploy solarpowered Street Charge stations in NYC, we go handson video

If you're lucky, your smartphone's battery can just make it through a full day, with the percentage meter dropping to the single digits as you finally get a chance to plug in. With 10 hours out and about, some handsets can't even last through dinner, though, and out of reach power outlets make juicing up at the restaurant a tad inconvenient. Beginning this summer, public power will be much more accessible in New York City. AT&T is partnering with Goal Zero to deploy Street Charge stations in a handful of parks and other locations around NYC. They'll be coming to Fort Green Park on June 18th, and will roll out to Brooklyn Bridge Park, Coney Island, Riverside Park, Rockaways, Summerstage in Central Park, Randall's Island, Governor's Island, Union Square, and Hudson River Park over the next few weeks. Each solar-powered pole, designed locally by Pensa, sports six USB connectors: for iPhones and iPads, there's 30-pin and Lightning plugs, Android and Windows Phone users can hook up to micro-USB, and everyone else can use their own cable with one of three female USB connectors.

There's three 15-watt solar panels and a 168 watt-hour battery, enabling each Street Charge to power up to six devices for several days without exposure to the sun. Sure, it's probably impractical to fill your gadget's cell completely, but if you have a few minutes to spare, you'll be able to juice up and go. You can plug in phones, tablets, cameras, or even a Pebble watch -- each cable can pump out up to two amps of 5V power, providing support for just about any USB-powered device. Each weatherproof unit will have AT&T branding, which seems reasonable, considering the carrier is footing the bill here. You should start seeing these pop up at several TBA locations in New York throughout the summer, with more stations to come if the trial's a success. Take a closer look in the hands-on video after the break.

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Source: http://www.engadget.com/2013/06/18/street-charge-nyc-hands-on/?utm_medium=feed&utm_source=Feed_Classic&utm_campaign=Engadget

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New way to improve antibiotic production

June 17, 2013 ? An antibiotic has been found to stimulate its own production. The findings, to be published in PNAS, could make it easier to scale up antibiotic production for commercialisation.

Scientists Dr Emma Sherwood and Professor Mervyn Bibb from the John Innes Centre were able to use their discovery of how the antibiotic is naturally produced to markedly increase the level of production.

"We have shown for the first time that an antibiotic with clinical potential can act as signalling molecule to trigger its own synthesis," said Professor Bibb.

The antibiotic called planosporicin is produced by a soil bacterium called Planomonospora alba. When nutrients become limited, a small amount of the antibiotic is produced. The antibiotic is then able to trigger a mechanism which coordinates its own production throughout the bacterial population resulting in high levels.

"A frequent stumbling block in developing a natural product for commercialisation is being able to provide enough material for clinical trials," said Professor Bibb.

"Our work shows with the right understanding it is possible to increase productivity very dramatically in a targeted and knowledge-based manner."

With knowledge of this signalling mechanism in hand, the scientists were able to increase production by overexpressing two positively acting regulatory genes and deleting one that acts negatively. Planosporicin is similar to the antibiotic NAI-107 that is about to enter clinical trials for Staphylococcus aureus (MRSA) and vancomycin-resistant enterococci (VRE) infections. The knowledge gained from this study is being used to increase NAI-107 production.

Commercial manufacturers of antibiotics may be able to use the results to reduce production times and therefore reduce costs. Bacteria often have to be grown for days and sometimes weeks before they start to make effective amounts of an antibiotic. Sherwood and Bibb were able to trigger production essentially from the beginning of growth.

The work was funded through JIC's core strategic grant from the Biotechnology and Biological Sciences Research Council.

Source: http://feeds.sciencedaily.com/~r/sciencedaily/~3/_AqpXED2Qnw/130617160900.htm

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Tuesday, June 18, 2013

White House says U.S. to resume talks with Taliban

NATO soldiers board a helicopter after a security handover ceremony outside Kabul, June 18, 2013. (Omar Sobhan??

Perhaps channeling Winston Churchill's famous warning about "the end of the beginning," the White House on Tuesday announced plans for direct talks with Afghanistan's Taliban militia, but it cautioned against expecting any quick breakthrough that might speed the end of America's longest war.

Among the likely items on the agenda: the return of U.S. Army Sgt. Bowe Bergdahl, held captive by the Taliban-allied Haqqani network since 2009.

U.S. and Taliban negotiators will hold formal talks "in a couple of days" in the Gulf state of Qatar, where the Taliban will officially open an office on Tuesday, U.S. officials said on a conference call with reporters. The negotiations are part of a diplomatic push to ease the American withdrawal by the end of 2014 and ensure the war-torn country does not serve as a springboard for attacks like the Sept. 11, 2001, terrorist strikes.

The White House announced the face-to-face negotiations even as Afghanistan President Hamid Karzai proclaimed that his war-torn country?s military and police had taken the lead from NATO forces. Karzai also announced the start of separate peace talks with the Taliban in Qatar while calling for them to move to Afghanistan as soon as possible.

"We need to be realistic. This is a new development, a potentially significant development. But peace is not at hand,? a senior administration official cautioned on the call, which was held on condition that none of the participants be named. The process of political reconciliation in Afghanistan "will certainly promise to be complex, long and messy," another top official said on the call.

In the U.S.-Taliban talks, Washington is likely to send James Dobbins, who took over May 10 as the State Department's special representative for Afghanistan and Pakistan. Officials on the call said they believed the Taliban negotiators would be "fully authorized" by the militia's leader, Mullah Omar, who has eluded capture since U.S. forces invaded Afghanistan in late 2001.

One of the officials said the Taliban would shortly release a statement saying ?that they oppose the use of Afghan soil to threaten other countries and, second, that they support an Afghan peace process," as long sought by the United States. The first part of the statement is meant to indicate that they will break with al-Qaida, the official said.

The Taliban and other insurgent groups must also commit to ending their attacks and accept Afghanistan's constitution?including protections for women and minority rights, the official said.

While the negotiations are a good first step on the path to peace, "there's no guarantee that this will happen quickly, if at all," the first official underlined. "The core of this process is not going to be the U.S. Taliban talks. Those can help advance the process, but the core of it is going to be negotiations among Afghans, and the level of trust on both sides is extremely low, as one would expect. So it's going to be a long, hard process if indeed it advances significantly at all."

And even as the talks continue, American forces will not "let up" in helping their Afghan counterparts or battling insurgents, one of the officials stressed.

Asked whether possible prisoner exchanges might be discussed, another official replied: "Clearly, we do want to get our soldier, Sgt. Bergdahl, back. And I would expect that detainee exchanges would be an item on the U.S.-Taliban agenda."

Still, "the first meeting is likely to be just an exchange of agendas rather than any substantive, detailed discussion. We?ll tell them what we want to talk about; they?ll tell us what they want to talk about; and we?ll both then adjourn and consult on next steps, and then have another meeting in a week or two later," one official said.

U.S. officials have long complained about Pakistan's role in at least tolerating the flow of insurgents and weapons into Afghanistan. But "Pakistan has been genuinely supportive of a peace process for Afghanistan," one official said."There has in the past been skepticism about their support, but in recent months I think we've seen evidence that there is genuine support and that they've employed their influence such as it is to encourage the Taliban to engage and to engage in this particular format."

The officials declined to spell out in detail how the talks might affect the timetable for withdrawing America's roughly 60,000 troops. While U.S. and NATO combat forces are due to depart by the end of 2014, putting their Afghan counterparts solely in charge, President Barack Obama is expected to leave a residual force to help train the local military and police and carry out counterterrorism operations.

"The levels and nature of our presence are obviously going to be influenced, on the one hand, by levels of violence in Afghanistan and, on the other hand, by the presence or absence of international terrorists in or around Afghanistan," one official said. "To the extent the talks contribute to diminishing violence and eliminating international terrorists in and around Afghanistan, that will have an impact on decisions regarding our future presence."

According to the independent organization iCasualties, 2,238 Americans have died in Afghanistan, including 64 in 2013.

Source: http://news.yahoo.com/blogs/ticket/u-resume-talks-taliban-days-white-house-150430725.html

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Military plans would put women in most combat jobs

FILE - In this Sept. 18, 2012 file photo, female soldiers from 1st Brigade Combat Team, 101st Airborne Division train on a firing range while testing new body armor in Fort Campbell, Ky., in preparation for their deployment to Afghanistan. Women may be able to begin training as Army Rangers by mid-2015, and as Navy SEALs a year later under broad plans Defense Secretary Chuck Hagel is approving that would slowly bring women into thousands of combat jobs, including those in the country?s elite special operations forces, according to details of the plans submitted to Hagel that were obtained by The Associated Press. (AP Photo/Mark Humphrey, File)

FILE - In this Sept. 18, 2012 file photo, female soldiers from 1st Brigade Combat Team, 101st Airborne Division train on a firing range while testing new body armor in Fort Campbell, Ky., in preparation for their deployment to Afghanistan. Women may be able to begin training as Army Rangers by mid-2015, and as Navy SEALs a year later under broad plans Defense Secretary Chuck Hagel is approving that would slowly bring women into thousands of combat jobs, including those in the country?s elite special operations forces, according to details of the plans submitted to Hagel that were obtained by The Associated Press. (AP Photo/Mark Humphrey, File)

FILE ? In this May 17, 2013 file photo Defense Secretary Chuck Hagel, left, and Chairman of the Joint Chiefs of Staff, Gen. Martin Dempsey take turns talking to media during a news conference at the Pentagon. Women may be able to begin training as Army Rangers by mid-2015, and as Navy SEALs a year later under broad plans Defense Secretary Chuck Hagel is approving that would slowly bring women into thousands of combat jobs, including those in the country?s elite special operations forces, according to details of the plans submitted to Hagel that were obtained by The Associated Press. (AP Photo/Carolyn Kaster, File)

(AP) ? Women may be able to start training as Army Rangers by mid-2015 and as Navy SEALs a year later under plans set to be announced by the Pentagon that would slowly bring women into thousands of combat jobs, including those in elite special operations forces.

Details of the plans were obtained by The Associated Press. They call for requiring women and men to meet the same physical and mental standards to quality for certain infantry, armor, commando and other front-line positions across the Army, Navy, Air Force and Marines. Defense Secretary Chuck Hagel reviewed the plans and has ordered the services to move ahead.

The move, expected to be announced Tuesday, follows revelations of a startling number of sexual assaults in the armed forces. Earlier this year, Joint Chiefs of Staff Chairman Gen. Martin Dempsey said the sexual assaults might be linked to the longstanding ban on women serving in combat because the disparity between the roles of men and women creates separate classes of personnel ? male "warriors" versus the rest of the force.

While the sexual assault problem is more complicated than that, he said, the disparity has created a psychology that lends itself to disrespect for women.

Under the schedules military leaders delivered to Hagel, the Army will develop standards by July 2015 to allow women to train and potentially serve as Rangers, and qualified women could begin training as Navy SEALS by March 2016 if senior leaders agree. Military leaders have suggested bringing senior women from the officer and enlisted ranks into special forces units first to ensure that younger, lower-ranking women have a support system to help them get through the transition.

The Navy intends to open up its Riverine force and begin training women next month, with the goal of assigning women to the units by October. While not part of the special operations forces, the coastal Riverine squadrons do close combat and security operations in small boats. The Navy plans to have studies finished by July 2014 on allowing women to serve as SEALs, and has set October 2015 as the date when women could begin Navy boot camp with the expressed intention of becoming SEALs eventually.

U.S. Special Operations Command is coordinating the matter of what commando jobs could be opened to women, what exceptions might be requested and when the transition would take place.

The proposals leave the door open for continued exclusion of women from some jobs, if research and testing find that women could not be successful in sufficient numbers, but the services would have to defend such decisions to top Pentagon leaders.

Army officials plan to complete gender-neutral standards for the Ranger course by July 2015. Army Rangers are one of the service's special operations units, but many soldiers who go through Ranger training and wear the coveted tab on their shoulders never actually serve in the 75th Ranger Regiment. To be considered a true Ranger, soldiers must serve in the regiment.

In January, then-Defense Secretary Leon Panetta and Joint Chiefs Chairman Gen. Martin Dempsey signed an order that wiped away generations of limits on where and how women could fight for their country. At the time, they asked the services to develop plans to set the change in motion.

The decision reflects a reality driven home by the wars in Iraq and Afghanistan, where battle lines were blurred and women were propelled into jobs as medics, military police and intelligence officers that were sometimes attached, but not formally assigned, to battalions. So, even though a woman could not serve officially as a battalion infantryman going out on patrol, she could fly a helicopter supporting the unit or be part of a team supplying medical aid if troops were injured.

Of the more than 6,700 U.S. service members who have been killed in Iraq and Afghanistan, about 150 have been women.

The order Panetta and Dempsey signed prohibits physical standards from being lowered simply to allow women to qualify for jobs closer to the battlefront. But the services are methodically reviewing and revising the standards for many jobs, including strength and stamina, in order to set minimum requirements for troops to meet regardless of their sex.

The military services are also working to determine the cost of opening certain jobs to women, particularly aboard a variety of Navy ships, including certain submarines, frigates, mine warfare and other smaller warships. Dozens of ships do not have adequate berthing or facilities for women to meet privacy needs, and would require design and construction changes.

Under a 1994 Pentagon policy, women were prohibited from being assigned to ground combat units below the brigade level. A brigade is roughly 3,500 troops split into several battalions of about 800 soldiers each. Historically, brigades were based farther from the front lines, and they often included top command and support staff.

Last year the military opened up about 14,500 combat positions to women, most of them in the Army, by allowing them to serve in many jobs at the battalion level. The January order lifted the last barrier to women serving in combat, but allows the services to argue to keep some jobs closed.

The bulk of the nearly 240,000 jobs currently closed to women are in the Army, including those in infantry, armor, combat engineer and artillery units that are often close to the battlefront. Similar jobs in the Marine Corps are also closed.

Army officials have laid out a rolling schedule of dates in 2015 to develop gender-neutral standards for specific jobs, beginning with July for engineers, followed by field artillery in March and the infantry and armor jobs no later than September.

Women make up about 14 percent of the 1.4 million active U.S. military personnel. More than 280,000 women have been sent to Iraq, Afghanistan or neighboring nations in support of the wars

Associated Press

Source: http://hosted2.ap.org/APDEFAULT/89ae8247abe8493fae24405546e9a1aa/Article_2013-06-17-Women%20in%20Combat/id-9605fd4f08be4284b98a4ecbd7e9497f

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Monday, June 17, 2013

Obama, Putin in stare-down over (no, not the Super Bowl ring) Syria war

So, did The Ring come up in the conversation?

Syria may have topped the agenda of Monday?s meeting between President Obama and Russian President Vladimir Putin ? the first between the two leaders in a year. Mr. Obama appears to have had no more success at convincing Mr. Putin to abandon his support for Syrian President Bashar al-Assad than Putin did in winning over Obama to his view that, in Syria, the West is wrong to demand that a legitimate government step down.

While the two presidents made it clear that their differences over a future path for Syria remain unresolved, the topic of Putin?s Super Bowl ring ? and whether or not the jewel, which now sits in a display case in the Kremlin library, was purloined ? received no public air time.

RECOMMENDED: Briefing Chemical weapons 101: Six facts about sarin and Syria?s stockpile

Airwaves buzzed over the weekend with reports that Putin might have come by the ring by swiping it from New England Patriots owner Robert Kraft. The odd tale appears to have started when Mr. Kraft, speaking last week at a charity gala in New York, said Putin pocketed the solid-gold bauble when Kraft showed it to him during a 2005 visit to St. Petersburg.

At the time, Kraft said he gave the ring to Putin. Now he seems to want it back.

It?s unclear if Obama acted as any kind of go-between in the Kraft-Putin dispute. Perhaps there was no time for such light-hearted fare as the two leaders took up their differing views on the Syrian civil war ? a conflict that threatens to engulf the region as it rages on.

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After their Monday afternoon t?te-?-t?te in Northern Ireland, where the two men gathered with other leaders for the annual G8 summit of industrialized countries, Obama said the US and Russia continue to have ?differing perspectives on the problem? of Syria?s 26-month-old civil war.

The two did try to paper over their differences, saying that both sides want to see a negotiated settlement to the conflict. Putin said he and Obama ?agreed to push the partiers to the negotiating table,? while Obama said the US and Russia agree on the need ?to try to resolve the issue through political means, if possible.?

A US-Russia plan for Syria peace talks in Geneva has stalled, with a conference originally set for May now envisioned for July at best.

But there were no signs that Obama was able to convince Putin that his decision, announced last week, to begin supplying US arms to Syria?s rebels will enhance the likelihood of bringing the warring factions to peace talks.

Obama took his case to Putin even as new polls in the US show that a majority of Americans don?t approve of the president?s about-face on getting the US more deeply involved in the Syrian conflict. A new Gallup poll finds that 54 percent disapprove of direct US military aid to the rebels, while 37 percent approve.

But a Pew poll suggests that a much higher percentage of Americans disapprove of the plan, announced by the White House last week. More than two-thirds of Americans ? 70 percent ? oppose the US and its allies supplying military assistance to the rebels, while just 20 percent approve of such a move, according to the Pew Research Center.

Moreover, the Pew survey finds that 6 in 10 Americans are skeptical that Syria?s opposition, if brought to power, would be any better than the current Assad government.

Appearing before the international press after two hours of talks, the American and Russian chiefs hardly looked like two leaders who had just enjoyed their time together.

Obama appeared to try to put more emphasis on their common ground, noting that both the US and Russia have an interest in ?securing chemical weapons and ensuring that they are neither used nor are ... subject to proliferation.?

Obama also took time in otherwise terse statements to announce a ?concrete outcome? of the two leaders? meeting: agreement on extension of US-Russia cooperation on nuclear security and nonproliferation known as the Nunn-Lugar program.

Other leaders attending the two-day summit were less diplomatic. Canada?s prime minister, Stephen Harper, said early that ?Mr. Putin and his government? were supporting ?the thugs of the Assad regime for their own reasons that I do not think are justifiable.?

As a result, he said, the G8 could have no common position on Syria. ?I don?t think we should fool ourselves,? he said. ?This is G7 plus one.?

RECOMMENDED: Briefing Chemical weapons 101: Six facts about sarin and Syria?s stockpile

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Source: http://news.yahoo.com/obama-putin-stare-down-over-no-not-super-223808405.html

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Mapping translation sites in the human genome

June 16, 2013 ? Because of their central importance to biology, proteins have been the focus of intense research, particularly the manner in which they are produced from genetically coded templates -- a process commonly known as translation. While the general mechanism of translation has been understood for some time, protein synthesis can initiate by more than one mechanism. One of the least well understood mechanisms is known as cap-independent translation.

Now, John Chaput and his colleagues at Arizona State University's Biodesign Institute have produced the first genome-wide investigation of cap-independent translation, identifying thousands of mRNA sequences that act as Translation Enhancing Elements (TEEs), which are RNA sequences upstream of the coding region that help recruit the ribosome to the translation start site.

The new study outlines a technique for mining whole genomes for sequences that initiate cap-independent translation within the vastness of the genome.

The research has important implications for the fundamental understanding of translation in living systems, as well as intriguing potential in the biomedical arena. (Many viral pathogens are known to use cap-independent translation to hijack and redirect cellular mechanisms to translate viral proteins.)

The lead author of the study is Brian P. Wellensiek, a senior scientist in Biodesign's Center for Evolutionary Medicine and Informatics. The group's results appear in the current issue of the journal Nature Methods.

During most protein synthesis in eukaryotic cells, cap-dependent translation dominates. The process begins after DNA is first transcribed into mRNA, with the aid of an enzyme polymerase. mRNA now forms the coded template from which the translated proteins will be generated. The mRNA code consists of sequences made from 4 nucleic acids, A, C, G & U, with each 3-letter grouping (known as a codon), corresponding to one amino acid in the protein being synthesized.

A key component in the translation process is the ribosome, which migrates along the single stranded mRNA, reading the codons as it goes. Before it can do this however, it must locate a special structure at the 5' end of the mRNA strand known as the cap. In normal cap-dependent translation, the ribosome is recruited to the 5' end of mRNA via a specialized cap-binding complex.

Cap-independent translation allows the ribosome to begin reading the mRNA message without having to first locate the 5' cap structure. Cap-independent translation occurs in eukaryotic cells during normal processes including mitosis and apoptosis (or programmed cell death). It is also a feature in many forms of viral translation, where the viral transcript is able to recruit the ribosome and co-opt its function to preferentially translate viral RNA.

In the current study, Chaput designed an in vitro selection strategy to identify human genome sequences that initiate cap-independent translation. The technique is able to select candidates from a pool of trillions of genomic fragments. Once a set of sequences was identified as translation enhancing elements, they were shown to function effectively in both cell-free and cellular translation systems.

As Chaput explains, most research on cap-independent translation has been conducted using RNA fragments derived from viruses. "These RNA molecules will fold into shapes that appear to mimic some of the initiation factors that that you would find in eukaryotic translation," he says. More recently, similar RNA molecules have been identified in cellular systems, though the sequences tend to be much shorter and function in a different manner.

Chaput's method of studying such sequences on a genome-wide scale involves first generating a DNA library of the entire human genome. Using enzymes, the genome is cut into random fragments of around 200 base pairs each. These sequences are then transcribed into mRNA.

Applying a technique known as mRNA display, the fragments are tagged in specific way, such that amino acid sequences resulting from successful translation events remain bound to the mRNA fragments that generated them. "Essentially, what we're doing is taking a library of human mRNA and tagging those sequences that act as translation enhancing elements," Chaput says. Those sequences bearing an attached peptide affinity tag can then be separated out from the remaining untranslated sequences, reverse transcribed, amplified using PCR technology and subjected to subsequent rounds of selection.

The sequences were later mapped onto the human genome. As expected, the complete library of sequences used at the start of the experiments mapped fairly evenly across the genome. But the sequences selected via mRNA display as translation enhancing elements tended to cluster in non-coding regions of the genome. The authors speculate that such sequences may have been evolutionarily selected against, as they have the potential to disrupt normal cap-dependent translation.

Roughly 20 percent of the translation enhancing elements functioned as internal ribosomal initiation sites, again turning up primarily in non-coding genomic regions. The origin of these sequences remains mysterious. It is conceivable that they were surreptitiously brought on board as a result of human interaction with different types of viruses.

Once Chaput's group had acquired a library of 250 distinct translation enhancing elements through selection using mRNA display, the sequences were screened for translation enhancing activity, which was quantified using a light based assay employing a luciferase reporter molecule.

By measuring levels of luciferase, the enhancement of each sequence could be assessed relative to background noise, with the better translation enhancing elements displaying 50-100 fold enhancement (and some as much as 1000-fold enhancement). The next step was to determine which of these sequences could function as internal ribosomal initiation sites.

To do this, the same 250 sequences were inserted into a vector bearing a hairpin structure. As Chaput explains: "If the ribosome latched onto the 5' end, it would hit that hairpin and would fall off. However if the ribosome skipped the hairpin and recognized the sequence on the other side of the hairpin independently and translated it, that's an indication that the sequence is functioning as an internal ribosomal initiation site." Both assays (for translation enhancement and internal ribosomal initiation) were validated under cell-free conditions and in human cells, using a vaccinia virus vector.

A study of this scope is possible thanks to innovative techniques for in vitro selection (such as mRNA display), as well as a revolutionary technology permitting massively parallel RNA sequencing (known as deep sequencing), which provides unprecedented speed and read accuracy.

Much remains to be learned about atypical translation processes. The mechanism of action for translation enhancing elements is still obscure, particularly in the case of internal ribosomal initiation sites. Similarly, the particular gene products that may result from cap-independent translation have yet to be identified and characterized.

Source: http://feeds.sciencedaily.com/~r/sciencedaily/~3/giXLkqwIWhM/130616155211.htm

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